DE-GROOT GROUP - LABORATORY EQUIPMENT LTD

Protein Crystallisation Plates

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CRYSTALQUICK Protein Crystallisation Plates

Greiner Bio-One

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Description

Protein Crystallisation

Numerous factors affect the crystallisation of proteins. Since the optimal crystallisation conditions generally cannot be predicted, alarge number of attempts is often necessary in order to determine and optimise the appropriate conditions. Protein crystallisation therefore still represents a major bottleneck in structure analysis. The use of high-throughput technologies, such as pipetting robots and standardised microplates, makes it possible to test a large number of crystallisation conditions in a short period of time and with relatively small amounts of protein. The CrystalStar™ product range from Greiner Bio-One is a family of crystallisation plates and accessories designed specifically for high-throughput crystallisation.

Format
We place great value on the suitability of our protein crystallisation plates for use with automated systems. Therefore, with the exception of Terasaki plates, all crystallisation plates have a footprint conforming to the ANSI/SBS 1-2004 Standard.

Material
All Greiner Bio-One protein crystallisation plates, with the exception of the LBR plates, are made from polystyrene. This is characterised by high clarity and excellent optical properties.

Hydrophobic Plates
Plates with a hydrophobic surface are particularly well suited for nanolitre crystallisation of membrane proteins. The surface properties of hydrophobic plates efficiently counteract the spreading of detergent-containing drops, respectively of drops with surfactant precipitants, such as MPD. Moreover, the meniscus of the screening solution in the reservoir is substantially reduced, so that contaminations through creeping of the screening solution into the crystallisation well are avoided.

LBR Plates
LBR (low birefringence) plates are specifically designed for the use of polarised light. LBR plates for sitting drop applications are made from polyolefin which is characterised by very low birefringence in comparison with polystyrene plates. Extreme transparency, high chemical resistance and low water absorption are further characteristics of LBR plates.

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