Advanced TC Vessels

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Greiner Bio-One

Catalogue number *
Description

Advanced TC™ Cell Culture Vessels

For the propagation of fastidious cells like primary or sensitive

cells as well as cells cultivated under restricted growth

conditions (serum-free or serum-reduced) Greiner Bio-One offers

the Advanced TC™ polymer modification. Based on an

innovative technique, the cell culture surface is modified to promote

cellular features and functions. Enhanced cell attachment

(Fig. 1) and higher proliferation rates (Fig. 2) improve and accelerate

cell expansion. Furthermore, the Advanced TC™ surface

facilitates consistent and homogenous cell attachment increasing

the overall cell yield and reducing cell loss, for example during

automated washing steps.

The positive effect on cell morphology is particularly apparent

during cultivation of sensitive cells (Fig. 3), serum deprivation

or after cellular stress induced by transfection or transduction

processes. Moreover, cells cultivated on the Advanced TC™

surface exhibit higher transgene activity after gene transfer/

insertion (Fig. 4).

Due to the production process, the modification of the polymer

assures consistant and reproducible product quality. Transport

and storage can be carried out at room temperature.

Applications:

Cultivation of fastidious and sensitive cells

Usage of serum-reduced or serum-free media

Differentiation of semi-adherent cells

Transfection

Transduction

Automation/High-throughput analysis

Advantages:

Improved cell adherence

Consistent cell attachment

Homogenous cell growth

In-vivo like morphology

Increased cell yield

Optimal cultivation conditions for sensitive cells

Permits usage of serum-reduced or serum-free media

Reduced cell loss due to (automated) washing steps

Improved assay consistency

Storage at room temperature

2-year shelf life
 
Application Notes
Catalogue Application Note Application Note

Greiner Bio-One

Catalogue number 627975
Description
CELLVIEW, CELLCULTURE DISH WITH GLASS BOTTOM, ADVANCED TC, 35 MM, 4 COMPARTMENTS, STERILE,
Description
Item No.: 627975
Product description: CELLVIEW CELL CULTURE DISH, PS, 35/10 MM,
GLASS BOTTOM, 4 COMPARTMENTS, ADVANCED TC,
STERILE, 10 PCS./BAG
Packaging weight: 0,40 kg
Packaging dimension: 195 x 142 x 122 mm
Packing unit: 40
Quantity per inner pack: 10
Sterile: sterile

Detailed information

  • CELLview - Cell Culture Dish with Glass Bottom

    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Glass bottom features:

                          - High transparent achromatic
                             borosilicate glass;
    hydrolytic
                             class 1 (DIN ISO 719)
                         
    - Glass thickness 175 µm +/- 15 µm
                         
    - Maximal spectral transmission; no
                            
    autofluorescence 

    • Advantages:

                          -Subdivided version enables
                            simultaneous
    multiplex analysis
                       
      - Embedded glass bottom for maximal
                            planarity

    • Number of compartments: 4
    • Diameter: 35 mm; height: 10 mm
    • Growth area: 1.9 cm²/compartment
    • Total volume: 1.5 ml/compartment
    • Working volume: 0.1 ml for seeding or staining only on glass area; 0.5 ml for cultivation in the complete compartment
    • Surface treatment: Advanced TC
    • Sterile

     

    Quantity per bag/case: 10/40

  • Videos
    Drug treatment during live cell imaging
    A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:
    • Video 1 - control (no drugs added)
      In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.

    • Video 2 - Nocodazole added, final concentration 10 µM
      Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.

    • Video 3 - Latrunculin B added, final concentration 1 μM
      Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi
      rearrangement was completed.

    • Video 4 - Brefeldin A added, final concentration 5 μg/ml
      Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.

    Performing these experiments in parallel in CELLview™ dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes.
    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 627965
    Description
    CELLVIEW, CELLCULTURE DISH WITH GLASS BOTTOM, ADVANCED TC, 35 MM, 1 COMPARTMENT, STERILE, 10 PIECES PER BAG
    Description
    Item No.: 627965
    Product description: CELLVIEW CELL CULTURE DISH, PS, 35/10 MM,
    GLASS BOTTOM, 1 COMPARTMENT, ADVANCED TC,
    STERILE, 10 PCS./BAG
    Packaging weight: 0,40 kg
    Packaging dimension: 195 x 142 x 122 mm
    Packing unit: 40
    Quantity per inner pack: 10
    Sterile: sterile

    Detailed information

    CELLview - Cell Culture Dish with Glass Bottom

    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Glass bottom features:

                          - High transparent achromatic
                             borosilicate glass;
    hydrolytic
                             class 1 (DIN ISO 719)
                     
       - Glass thickness 175 µm +/- 15 µm
                         - Maximal spectral transmission; no
                          
    autofluorescence 

    • Advantages:

                           -Subdivided version enables
                            simultaneous
    multiplex analysis
                        
     - Embedded glass bottom for maximal
                            planarity

    • Number of compartments: 1
    • Diameter: 35 mm; height: 10 mm
    • Growth area: 8.7 cm²
    • Total volume: 10 ml
    • Working volume: 5 ml
    • Surface treatment: Advanced TC
    • Sterile

     

    Quantity per bag/case: 10/40

     

    Videos
    Drug treatment during live cell imaging
    A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:

    • Video 1 - control (no drugs added)
      In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.

    • Video 2 - Nocodazole added, final concentration 10 µM
      Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.

    • Video 3 - Latrunculin B added, final concentration 1 μM
      Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi
      rearrangement was completed.

    • Video 4 - Brefeldin A added, final concentration 5 μg/ml
      Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.

    Performing these experiments in parallel in CELLview™ dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes. ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 627960
    Description
    CELL CULTURE DISH, 35x10 MM, STERILE, ADVANCED TC, 10 PIECES PER BAG
    Description
    Item No.: 627960
    Product description: CELLVIEW CELL CULTURE DISH, PS, 35/10 MM, VENTS,
    ADVANCED TC, STERILE, 10 PCS./BAG
    Packaging weight: 2,80 kg
    Packaging dimension: 295 x 195 x 370 mm
    Packing unit: 740
    Quantity per inner pack: 10
    Sterile: sterile

    Detailed information

    Advanced TC™ - Cell Culture Dish

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Advantages:
                 - Improved cell adherence
                 - Consistent cell attachment
                 - Homogenous cell growth
                 - In-vivo like morphology
                 - Increased cell yield
                 - Optimal cultivation conditions for sensitive
                   cells
                 - Permits usage of serum-reduced or serum-
                   free media
                 - Reduced cell loss due to (automated)
                   washing
     steps
                 - Improved assay consistency
                 - Storage at room temperature
                 - 2-year shelf-life
    • Diameter: 35 mm; height: 10 mm
    • Growth area: 8.7 cm²
    • Total Volume: 10 ml
    • Working volume: 5 ml
    • With vents 
    • Sterile

     

    Quantity per bag/case: 10/740

    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 628960
    Description
    CELL CULTURE DISH, 60x15 MM, STERILE, ADVANCED TC, 10 PIECES PER BAG
    Description
    Item No.: 628960
    Product description: CELL CULTURE DISH, PS, 60/15 MM, VENTS,
    ADVANCED TC, STERILE, 10 PCS./BAG
    Packaging weight: 5,30 kg
    Packaging dimension: 295 x 390 x 365 mm
    Packing unit: 600
    Quantity per inner pack: 10
    Sterile: sterile

    Detailed information

    Advanced TC™ - Cell Culture Dish

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Advantages:
                 - Improved cell adherence
                 - Consistent cell attachment
                 - Homogenous cell growth
                 - In-vivo like morphology
                 - Increased cell yield
                 - Optimal cultivation conditions for sensitive
                   cells
                 - Permits usage of serum-reduced or serum-
                   free media
                 - Reduced cell loss due to (automated)
                   washing
     steps
                 - Improved assay consistency
                 - Storage at room temperature
                 - 2-year shelf-life
    • Diameter: 60 mm; height: 15 mm
    • Growth area: 21 cm²
    • Total Volume: 28 ml
    • Working volume: 6 ml - 7 ml
    • With vents 
    • Sterile

     

    Quantity per bag/case: 10/600

    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 639960
    Description
    CELL CULTURE DISH, 145x20 MM, STERILE, ADVANCED TC, 5/120 BAG/CASE
    Description
    Item No.: 639960
    Product description: CELL CULTURE DISH, PS, 145/20 MM,
    VENTS, ADVANCED TC, STERILE, 5 PCS./BAG
    Packaging weight: 6,90 kg
    Packaging dimension: 590 x 330 x 360 mm
    Packing unit: 120
    Quantity per inner pack: 5
    Sterile: sterile

    Detailed information

    Advanced TC™ - Cell Culture Dish

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Advantages:
                 - Improved cell adherence
                 - Consistent cell attachment
                 - Homogenous cell growth
                 - In-vivo like morphology
                 - Increased cell yield
                 - Optimal cultivation conditions for sensitive
                   cells
                 - Permits usage of serum-reduced or serum-
                   free media
                 - Reduced cell loss due to (automated)
                   washing
     steps
                 - Improved assay consistency
                 - Storage at room temperature
                 - 2-year shelf-life
    • Diameter: 145 mm; height: 20 mm
    • Growth area: 143 cm²
    • Total Volume: 240 ml
    • Working volume: 25 ml - 27 ml
    • With vents 
    • Sterile

     

    Quantity per bag/case: 5/120

    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 655980
    Description
    MICROPLATE, 96 WELL, WITH LID, F-BOTTOM/CHIMNEY, TRANSPARENT, STERILE, ADVANCED TC SURFACE,  INDIVIDUALLY PACKED
    Description
    Item No.: 655980
    Product description: CELL CULTURE MICROPLATE, 96 WELL, PS, F-BOTTOM
    (CHIMNEY WELL), CLEAR, ADVANCED TC, LID WITH
    CONDENSATION RINGS, STERILE, SINGLE PACKED
    Packaging weight: 6,50 kg
    Packaging dimension: 500 x 346 x 176 mm
    Packing unit: 100
    Quantity per inner pack: 1
    Sterile: sterile

    Detailed information

  • Advanced TC™ - 96 Well Cell Culture Microplate, clear

    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Advantages:
                 - Improved cell adherence
                 - Consistent cell attachment
                 - Homogenous cell growth
                 - In-vivo like morphology
                 - Increased cell yield
                 - Optimal cultivation conditions for sensitive
                   cells
                 - Permits usage of serum-reduced or serum-
                   free media
                 - Reduced cell loss due to (automated)
                   washing
     steps
                 - Improved assay consistency
                 - Storage at room temperature
                 - 2-year shelf-life
    • Solid bottom
    • Well profile: F-bottom/chimney well (flat bottom)
    • Growth area per well: 34 mm²
    • Working volume per well: 25 µl - 340 µl
    • Lid with condensation rings
    • Sterile

     

    Quantity per bag/case: 1/100

    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 655982
    Description
    MICROPLATE, 96 WELL, WITH LID, F-BOTTOM/CHIMNEY, TRANSPARENT, STERILE, ADVANCED TC SURFACE, 10 PIECES PER BAG
    Description
    Item No.: 655982
    Product description: CELL CULTURE MICROPLATE, 96 WELL, PS, F-BOTTOM
    (CHIMNEY WELL), CLEAR, ADVANCED TC, LID WITH
    CONDENSATION RINGS, STERILE, 10 PCS./BAG
    Packaging weight: 11,10 kg
    Packaging dimension: 295 x 390 x 365 mm
    Packing unit: 160
    Quantity per inner pack: 10
    Sterile: sterile

    Detailed information

    Advanced TC 96 Well Cell Culture Microplates

    •   Applications:
      • Cultivation of fastidious and sensitive cells
      • Usage of serum-reduced or serum-free media
      • Differentiation of semi-adherent cells
      • Transfection
      • Transduction
      • Automation/High-throughput analysis
    • Advantages:
      • Improved cell adherence
      • Consistent cell attachment
      • Homogenous cell growth
      • In-vivo like morphology
      • Increased cell yield
      • Optimal cultivation conditions for sensitive cells
      • Permits usage of serum-reduced or serum-free media
      • Reduced cell loss due to (automated) washing steps
      • Improved assay consistency
      • Storage at room temperature
      • 2-year shelf life
    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic
    • Non-cytotoxic
    • Well format: 96 well
    • Well profile: F-bottom/chimney well
    • Solid bottom
    • Colour: clear
    • Growth area per well: 34 mm²
    • Working volume per well: 25 – 340 μl
    • Advanced TC
    • Sterile
    •  With lid with condensation rings

     
    Quantity per bag/case: 10/160

    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 655983
    Description
    MICROPLATE, 96 WELL, WITH LID, F-BOTTOM/CHIMNEY, µCLEAR, WHITE, ADVANCED TC SURFACE, STERILE,
    Description
    Item No.: 655983
    Product description: CELL CULTURE MICROPLATE, 96 WELL, PS, F-BOTTOM
    (CHIMNEY WELL), µCLEAR®, WHITE, ADVANCED TC,
    LID WITH CONDENSATION RINGS, STERILE, 8 PCS./BAG
    Packaging weight: 2,40 kg
    Packaging dimension: 335 x 250 x 165 mm
    Packing unit: 32
    Quantity per inner pack: 8
    Sterile: sterile

    Detailed information

    Advanced TC™- 96 Well Cell Culture Microplate, white

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Advantages:
                 - Improved cell adherence
                 - Consistent cell attachment
                 - Homogenous cell growth
                 - In-vivo like morphology
                 - Increased cell yield
                 - Optimal cultivation conditions for sensitive
                   cells
                 - Permits usage of serum-reduced or serum-
                   free media
                 - Reduced cell loss due to (automated)
                   washing
     steps
                 - Improved assay consistency
                 - Storage at room temperature
                 - 2-year shelf-life
    • µClear® bottom
    • Well profile: F-bottom/chimney well (flat bottom)
    • Growth area per well: 34 mm²
    • Working volume per well: 25 µl - 340 µl
    • Lid with condensation rings
    • Sterile

     

    Quantity per bag/case: 8/32

    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 655986
    Description
    MICROPLATE, 96 WELL, WITH LID, F-BOTTOM/CHIMNEY, µCLEAR, BLACK, ADVANCED TC SURFACE, STERILE,
    Description
    Item No.: 655986
    Product description: CELL CULTURE MICROPLATE, 96 WELL, PS, F-BOTTOM
    (CHIMNEY WELL), µCLEAR®, BLACK, ADVANCED TC,
    LID WITH CONDENSATION RINGS, STERILE, 8 PCS./BAG
    Packaging weight: 2,40 kg
    Packaging dimension: 335 x 250 x 165 mm
    Packing unit: 32
    Quantity per inner pack: 8
    Sterile: sterile

    Detailed information

    Advanced TC™- 96 Well Cell Culture Microplate, black

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Advantages:
                 - Improved cell adherence
                 - Consistent cell attachment
                 - Homogenous cell growth
                 - In-vivo like morphology
                 - Increased cell yield
                 - Optimal cultivation conditions for sensitive
                   cells
                 - Permits usage of serum-reduced or serum-
                   free media
                 - Reduced cell loss due to (automated)
                   washing
     steps
                 - Improved assay consistency
                 - Storage at room temperature
                 - 2-year shelf-life
    • µClear® bottom
    • Well profile: F-bottom/chimney well (flat bottom)
    • Growth area per well: 34 mm²
    • Working volume per well: 25 µl - 340 µl
    • Lid with condensation rings
    • Sterile

     

    Quantity per bag/case: 8/32

    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 657960
    Description
    MULTIWELL PLATE, 6 WELL, WITH LID, STERILE, ADVANCED TC SURFACE, INDIVIDUALLY PACKED
    Description
    Item No.: 657960
    Product description: CELL CULTURE MULTIWELL PLATE, 6 WELL, PS, CLEAR,
    ADVANCED TC, LID WITH CONDENSATION RINGS,
    STERILE, SINGLE PACKED
    Packaging weight: 6,10 kg
    Packaging dimension: 550 x 300 x 245 mm
    Packing unit: 100
    Sterile: sterile

    Detailed information

    Advanced TC™ - 6 Well Cell Culture Multiwell Plate

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Advantages:
                 - Improved cell adherence
                 - Consistent cell attachment
                 - Homogenous cell growth
                 - In-vivo like morphology
                 - Increased cell yield
                 - Optimal cultivation conditions for sensitive
                   cells
                 - Permits usage of serum-reduced or serum-
                   free media
                 - Reduced cell loss due to (automated)
                   washing
     steps
                 - Improved assay consistency
                 - Storage at room temperature
                 - 2-year shelf-life
    • Growth area per well: 9.6 cm²
    • Working volume per well: 2 ml - 5 ml
    • Lid with condensation rings
    • Sterile

     

    Quantity per bag/case: 1/100

    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 658970
    Description
    CELLCULTURE FLASK, 250 ML, 75 CM², STERILE, ADVANCED TC, STANDARD SCREW CAP, 5 PIECES PER BAG
    Description
    Item No.: 658970
    Product description: CELL CULTURE FLASK, 250 ML, 75 CM², PS,
    ADVANCED TC, BLUE STANDARD SCREW CAP, CLEAR,
    STERILE, 5 PCS./BAG
    Packaging weight: 6,70 kg
    Packaging dimension: 590 x 330 x 360 mm
    Packing unit: 120

    Detailed information

    Advanced TC™ Standard Cap Cell Culture Flask

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-Pyrogenic, non-cytotoxic
    • Applications
      - Cultivation of fastidious and sensitive cells
      - Usage of serum-reduced or serum-free
         media
      - Differentiation of semi-adherent cells
      - Transfection
      - Transduction
      - Automation/High-throughput analysis
    • Advantages
      - Improved cell adherence
      - Consistent cell attachment
      - Homogenous cell growth
      - In-vivo like morphology
      - Increased cell yield
      - Optimal cultivation conditions for sensitive
         cells
      - Permits usage of serum-reduced or
         serum-free media
      - Reduced cell los due to (automated) washing
        steps
      - Improved assay consistency
      - Storage at room temperature
      - 2-year shelf life
    • Growth area: 75 cm²
    • Total Volume: 250 ml
    • Working volume: 15 ml - 38 ml
    • With blue standard screw cap
    • Sterile

    Quantity per bag/case: 5/120 ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 658975
    Description
    FILTERTOP CELLCULTURE FLASK, 250 ML, 75 CM², STERILE, ADVANCED TC, FILTER SCREW CAP, 5 PIECES PER BAG
    Description
    Item No.: 658975
    Product description: CELL CULTURE FLASK, 250 ML, 75 CM², PS,
    ADVANCED TC, BLUE FILTER SCREW CAP, CLEAR,
    STERILE, 5 PCS./BAG
    Packaging weight: 6,70 kg
    Packaging dimension: 590 x 330 x 360 mm
    Packing unit: 120
    Sterile: sterile

    Detailed information

    Advanced TC™ Filter Cap Cell Culture Flask

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-Pyrogenic, non-cytotoxic
    • Applications
      - Cultivation of fastidious and sensitive cells
      - Usage of serum-reduced or serum-free
         media
      - Differentiation of semi-adherent cells
      - Transfection
      - Transduction
      - Automation/High-throughput analysis
    • Advantages
      - Improved cell adherence
      - Consistent cell attachment
      - Homogenous cell growth
      - In-vivo like morphology
      - Increased cell yield
      - Optimal cultivation conditions for sensitive
         cells
      - Permits usage of serum-reduced or
         serum-free media
      - Reduced cell los due to (automated) washing
        steps
      - Improved assay consistency
      - Storage at room temperature
      - 2-year shelf life
    • Growth area: 75 cm²
    • Total Volume: 250 ml
    • Working volume: 15 ml - 38 ml
    • With blue filter screw cap
    • Sterile

    Quantity per bag/case: 5/120 ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 660960
    Description
    CELLCULTURE FLASK, 550 ML, 175 CM², STERILE, ADVANCED TC, STANDARD SCREW CAP, 5/BAG
    Description
    Item No.: 660960
    Product description: CELL CULTURE FLASK, 550 ML, 175 CM², PS,
    ADVANCED TC, BLUE STANDARD SCREW CAP,
    CLEAR, FLAT FLASK DESIGN, STERILE,
    5 PCS./BAG
    Packaging weight: 6,80 kg
    Packaging dimension: 595 x 395 x 250 mm
    Packing unit: 50
    Quantity per inner pack: 5
    Sterile: sterile

    Detailed information

    Advanced TC™ Standard Cap Cell Culture Flask - flat flask design

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-Pyrogenic, non-cytotoxic
    • Applications
      - Cultivation of fastidious and sensitive cells
      - Usage of serum-reduced or serum-free
         media
      - Differentiation of semi-adherent cells
      - Transfection
      - Transduction
      - Automation/High-throughput analysis
    • Advantages
      - Improved cell adherence
      - Consistent cell attachment
      - Homogenous cell growth
      - In-vivo like morphology
      - Increased cell yield
      - Optimal cultivation conditions for sensitive
         cells
      - Permits usage of serum-reduced or
         serum-free media
      - Reduced cell los due to (automated) washing
        steps
      - Improved assay consistency
      - Storage at room temperature
      - 2-year shelf life
    • Growth area: 175 cm²
    • Total Volume: 550 ml
    • Working volume: 20 ml - 45 ml
    • With blue standard screw cap
    • Sterile

    Quantity per bag/case: 5/50 ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 660975
    Description
    FILTERTOP CELLCULTURE FLASK, 550 ML, 175 CM², STERILE, ADVANCED TC, FILTER SCREW CAP, 5/50 BAG/CASE
    Description
    Item No.: 660975
    Product description: CELL CULTURE FLASK, 550 ML, 175 CM², PS,
    ADVANCED TC, BLUE FILTER SCREW CAP, CLEAR,
    FLAT FLASK DESIGN, STERILE, 5 PCS./BAG
    Packaging weight: 6,70 kg
    Packaging dimension: 595 x 395 x 250 mm
    Packing unit: 50
    Sterile: sterile

    Detailed information

    Advanced TC™ Filter Cap Cell Culture Flask - flat flask design

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-Pyrogenic, non-cytotoxic
    • Applications
      - Cultivation of fastidious and sensitive cells
      - Usage of serum-reduced or serum-free
         media
      - Differentiation of semi-adherent cells
      - Transfection
      - Transduction
      - Automation/High-throughput analysis
    • Advantages
      - Improved cell adherence
      - Consistent cell attachment
      - Homogenous cell growth
      - In-vivo like morphology
      - Increased cell yield
      - Optimal cultivation conditions for sensitive
         cells
      - Permits usage of serum-reduced or
         serum-free media
      - Reduced cell los due to (automated) washing
        steps
      - Improved assay consistency
      - Storage at room temperature
      - 2-year shelf life
    • Growth area: 175 cm²
    • Total Volume: 550 ml
    • Working volume: 20 ml - 45 ml
    • With blue filter screw cap
    • Sterile

    Quantity per bag/case: 5/50 ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 661960
    Description
    CELLCULTURE FLASK, 650 ML, 175 CM², STERILE, ADVANCED TC, STANDARD SCREW CAP, 4/40 BAG/CASE
    Description
    Item No.: 661960
    Product description: CELL CULTURE FLASK, 650 ML, 175 CM², PS,
    ADVANCED TC, BLUE STANDARD SCREW CAP,
    CLEAR, HIGH FLASK DESIGN, STERILE, 4 PCS./BAG
    Packaging weight: 6,00 kg
    Packaging dimension: 595 x 395 x 250 mm
    Packing unit: 40
    Quantity per inner pack: 4
    Sterile: sterile

    Detailed information

    Advanced TC™ Standard Cap Cell Culture Flask - high flask design

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-Pyrogenic, non-cytotoxic
    • Applications
      - Cultivation of fastidious and sensitive cells
      - Usage of serum-reduced or serum-free
         media
      - Differentiation of semi-adherent cells
      - Transfection
      - Transduction
      - Automation/High-throughput analysis
    • Advantages
      - Improved cell adherence
      - Consistent cell attachment
      - Homogenous cell growth
      - In-vivo like morphology
      - Increased cell yield
      - Optimal cultivation conditions for sensitive
         cells
      - Permits usage of serum-reduced or
         serum-free media
      - Reduced cell los due to (automated) washing
        steps
      - Improved assay consistency
      - Storage at room temperature
      - 2-year shelf life
    • Growth area: 175 cm²
    • Total Volume: 650 ml
    • Working volume: 20 ml - 85 ml
    • With blue standard screw cap
    • Sterile

    Quantity per bag/case: 4/40 ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 661975
    Description
    FILTERTOP CELLCULTURE FLASK, 650 ML, 175 CM², STERILE, ADVANCED TC, FILTER SCREW CAP, 4/40 BAG/CASE
    Description
    Item No.: 661975
    Product description: CELL CULTURE FLASK, 650 ML, 175 CM², PS,
    ADVANCED TC, BLUE FILTER SCREW CAP,
    CLEAR, HIGH FLASK DESIGN, STERILE, 4 PCS./BAG
    Packaging weight: 6,20 kg
    Packaging dimension: 595 x 395 x 250 mm
    Packing unit: 40
    Quantity per inner pack: 4
    Sterile: sterile

    Detailed information

    Advanced TC™ Filter Cap Cell Culture Flask - high flask design

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-Pyrogenic, non-cytotoxic
    • Applications
      - Cultivation of fastidious and sensitive cells
      - Usage of serum-reduced or serum-free
         media
      - Differentiation of semi-adherent cells
      - Transfection
      - Transduction
      - Automation/High-throughput analysis
    • Advantages
      - Improved cell adherence
      - Consistent cell attachment
      - Homogenous cell growth
      - In-vivo like morphology
      - Increased cell yield
      - Optimal cultivation conditions for sensitive
         cells
      - Permits usage of serum-reduced or
         serum-free media
      - Reduced cell los due to (automated) washing
        steps
      - Improved assay consistency
      - Storage at room temperature
      - 2-year shelf life
    • Growth area: 175 cm²
    • Total Volume: 650 ml
    • Working volume: 20 ml - 85 ml
    • With blue filter screw cap
    • Sterile

    Quantity per bag/case: 4/40 ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 662960
    Description
    MULTIWELL PLATE, 24 WELL, WITH LID, STERILE, ADVANCED TC SURFACE, INDIVIDUALLY PACKED
    Description
    Item No.: 662960
    Product description: CELL CULTURE MULTIWELL PLATE, 24 WELL, PS,
    CLEAR, ADVANCED TC, LID WITH CONDENSATION
    RINGS, STERILE, SINGLE PACKED
    Packaging weight: 7,30 kg
    Packaging dimension: 550 x 300 x 245 mm
    Packing unit: 100
    Sterile: sterile

    Detailed information

    Advanced TC™ - 24 Well Cell Culture Multiwell Plate

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Advantages:
                 - Improved cell adherence
                 - Consistent cell attachment
                 - Homogenous cell growth
                 - In-vivo like morphology
                 - Increased cell yield
                 - Optimal cultivation conditions for sensitive
                   cells
                 - Permits usage of serum-reduced or serum-
                   free media
                 - Reduced cell loss due to (automated)
                   washing
     steps
                 - Improved assay consistency
                 - Storage at room temperature
                 - 2-year shelf-life
    • Growth area per well: 1.9 cm²
    • Working volume per well: 0.5 ml - 1.5 ml
    • Lid with condensation rings
    • Sterile

     

    Quantity per bag/case: 1/100

    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 664960
    Description
    CELL CULTURE DISH, 100x20 MM, STERILE, ADVANCED TC, 15 PIECES PER BAG
    Description
    Item No.: 664960
    Product description: CELL CULTURE DISH, PS, 100/20 MM, VENTS,
    ADVANCED TC, STERILE, 15 PCS./BAG
    Packaging weight: 8,90 kg
    Packaging dimension: 595 x 395 x 370 mm
    Packing unit: 360
    Sterile: sterile

    Detailed information

    Advanced TC™ - Cell Culture Dish

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Advantages:
                 - Improved cell adherence
                 - Consistent cell attachment
                 - Homogenous cell growth
                 - In-vivo like morphology
                 - Increased cell yield
                 - Optimal cultivation conditions for sensitive
                   cells
                 - Permits usage of serum-reduced or serum-
                   free media
                 - Reduced cell loss due to (automated)
                   washing
     steps
                 - Improved assay consistency
                 - Storage at room temperature
                 - 2-year shelf-life
    • Diameter: 100 mm; height: 20 mm
    • Growth area: 58 cm²
    • Total Volume: 100 ml
    • Working volume: 16 ml - 17 ml
    • With vents 
    • Sterile

     

    Quantity per bag/case: 15/360

    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)

    Greiner Bio-One

    Catalogue number 665980
    Description
    MULTIWELL PLATE, 12 WELL, WITH LID, STERILE, ADVANCED TC SURFACE, INDIVIDUALLY PACKED
    Description
    Item No.: 665980
    Product description: CELL CULTURE MULTIWELL PLATE, 12 WELL, PS,
    CLEAR, ADVANCED TC, LID WITH CONDENSATION
    RINGS, STERILE, SINGLE PACKED
    Packaging weight: 6,60 kg
    Packaging dimension: 550 x 300 x 245 mm
    Packing unit: 100
    Sterile: sterile

    Detailed information

    Advanced TC™ - 12 Well Cell Culture Multiwell Plate

    • Advanced TC polymer modification for fastidious or sensitive adherent cells
    • Free of detectable DNase, RNase, human DNA
    • Non-pyrogenic, non-cytotoxic
    • Advantages:
                 - Improved cell adherence
                 - Consistent cell attachment
                 - Homogenous cell growth
                 - In-vivo like morphology
                 - Increased cell yield
                 - Optimal cultivation conditions for sensitive
                   cells
                 - Permits usage of serum-reduced or serum-
                   free media
                 - Reduced cell loss due to (automated)
                   washing
     steps
                 - Improved assay consistency
                 - Storage at room temperature
                 - 2-year shelf-life
    • Growth area per well: 3.9 cm²
    • Working volume per well: 2 ml - 4 ml
    • Lid with condensation rings
    • Sterile

     

    Quantity per bag/case: 1/100

    ITEM AT GREINER'S WEBSITE
    Customer Drawings Data Sheets Related Items (pdf)
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