Description
Item No.:	627860
Product description:	CELLVIEW CELL CULTURE DISH, PS, 35/10 MM, GLASS BOTTOM, 1 COMPARTMENT, TC, STERILE, 10 PCS./BAG
Packaging weight:	0,34 kg
Packaging dimension:	195 x 145 x 125 mm
Packing unit:	40
Quantity per inner pack:	10
Sterile:	sterile

Detailed information

CELLview™ - Cell Culture Dish with Glass Bottom

• Free of detectable DNase, RNase, human DNA
• Non-pyrogenic, non-cytotoxic
• Glass bottom features:

• Advantages:

                      -Subdivided version enables
                        simultaneous multiplex analysis

                      - Embedded glass bottom for maximal
                        planarity

• Number of compartments: 1
• Diameter: 35 mm; height: 10 mm
• Growth area: 8.7 cm²
• Total volume: 10 ml
• Working volume: 5 ml
• Surface treatment: TC
• Sterile

 

Quantity per bag/case: 10/40

 

Videos
Drug treatment during live cell imaging
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:

• Video 1 - control (no drugs added)
  In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.
  
  
• Video 2 - Nocodazole added, final concentration 10 µM
  Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.
  
  
• Video 3 - Latrunculin B added, final concentration 1 μM
  Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi
  rearrangement was completed.
  
  
• Video 4 - Brefeldin A added, final concentration 5 μg/ml
  Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.

Performing these experiments in parallel in CELLview™ dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes.
ITEM AT GREINER'S WEBSITE

Description
Item No.:	627861
Product description:	CELLVIEW CELL CULTURE DISH, PS, 35/10 MM, GLASS BOTTOM, 1 COMPARTMENT, STERILE, 10 PCS./BAG
Packaging weight:	0,30 kg
Packaging dimension:	195 x 145 x 125 mm
Packing unit:	40
Quantity per inner pack:	10
Sterile:	sterile

Detailed information

CELLview™ - Cell Culture Dish with Glass Bottom

• Free of detectable DNase, RNase, human DNA
• Non-pyrogenic, non-cytotoxic
• Glass bottom features:

                      - High transparent achromatic
                         borosilicate glass; hydrolytic
                         class 1 (DIN ISO 719)
                     - Glass thickness 175 µm +/- 15 µm
                     - Maximal spectral transmission; no
                       autofluorescence 

• Advantages:

                       -Subdivided version enables
                        simultaneous multiplex analysis
                      - Embedded glass bottom for maximal
                        planarity

• Number of compartments: 1
• Diameter: 35 mm; height: 10 mm
• Growth area: 8.7 cm²
• Total volume: 10 ml
• Working volume: 5 ml
• Sterile

 

Quantity per bag/case: 10/40

 

Videos
Drug treatment during live cell imaging
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:

• Video 1 - control (no drugs added)
  In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.
  
  
• Video 2 - Nocodazole added, final concentration 10 µM
  Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.
  
  
• Video 3 - Latrunculin B added, final concentration 1 μM
  Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi
  rearrangement was completed.
  
  
• Video 4 - Brefeldin A added, final concentration 5 μg/ml
  Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.

Performing these experiments in parallel in CELLview™ dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes.


ITEM AT GREINER'S WEBSITE

Description
Item No.:	627870
Product description:	CELLVIEW CELL CULTURE DISH, PS, 35/10 MM, GLASS BOTTOM, 4 COMPARTMENTS, TC, STERILE, 10 PCS./BAG
Packaging weight:	0,40 kg
Packaging dimension:	195 x 142 x 122 mm
Packing unit:	40
Quantity per inner pack:	10
Sterile:	sterile

Detailed information

CELLview™ - Cell Culture Dish with Glass Bottom

• Free of detectable DNase, RNase, human DNA
• Non-pyrogenic, non-cytotoxic
• Glass bottom features:

                      - High transparent achromatic
                         borosilicate glass; hydrolytic
                         class 1 (DIN ISO 719)
                      - Glass thickness 175 µm +/- 15 µm
                      - Maximal spectral transmission; no
                         autofluorescence 

• Advantages:

                      -Subdivided version enables
                        simultaneous multiplex analysis
                     - Embedded glass bottom for maximal
                        planarity

• Number of compartments: 4
• Diameter: 35 mm; height: 10 mm
• Growth area: 1.9 cm²/compartment
• Total volume: 1.5 ml/compartment
• Working volume: 0.1 ml for seeding or staining only on glass area; 0.5 ml for cultivation in the complete compartment
• Surface treatment: TC
• Sterile

 

Quantity per bag/case: 10/40

Videos
Drug treatment during live cell imaging
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:

• Video 1 - control (no drugs added)
  In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.
  
  
• Video 2 - Nocodazole added, final concentration 10 µM
  Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.
  
  
• Video 3 - Latrunculin B added, final concentration 1 μM
  Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi
  rearrangement was completed.
  
  
• Video 4 - Brefeldin A added, final concentration 5 μg/ml
  Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.

Performing these experiments in parallel in CELLview™ dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes.

ITEM AT GREINER'S WEBSITE

Description
Item No.:	627871
Product description:	CELLVIEW CELL CULTURE DISH, PS, 35/10 MM, GLASS BOTTOM, 4 COMPARTMENTS, STERILE, 10 PCS./BAG
Packaging weight:	0,40 kg
Packaging dimension:	195 x 142 x 122 mm
Packing unit:	40
Quantity per inner pack:	10
Sterile:	sterile

Detailed information

CELLview™ - Cell Culture Dish with Glass Bottom

• Free of detectable DNase, RNase, human DNA
• Non-pyrogenic, non-cytotoxic
• Glass bottom features:

                      - High transparent achromatic
                         borosilicate glass; hydrolytic
                         class 1 (DIN ISO 719)
                      - Glass thickness 175 µm +/- 15 µm
                      - Maximal spectral transmission; no
                         autofluorescence 

• Advantages:

                      -Subdivided version enables
                        simultaneous multiplex analysis
                     - Embedded glass bottom for maximal
                        planarity

• Number of compartments: 4
• Diameter: 35 mm; height: 10 mm
• Growth area: 1.9 cm²/compartment
• Total volume: 1.5 ml/compartment
• Working volume: 0.1 ml for seeding or staining only on glass area; 0.5 ml for cultivation in the complete compartment
• Sterile

 

Quantity per bag/case: 10/40

 

Videos
Drug treatment during live cell imaging
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:

• Video 1 - control (no drugs added)
  In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.
  
  
• Video 2 - Nocodazole added, final concentration 10 µM
  Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.
  
  
• Video 3 - Latrunculin B added, final concentration 1 μM
  Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi
  rearrangement was completed.
  
  
• Video 4 - Brefeldin A added, final concentration 5 μg/ml
  Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.

Performing these experiments in parallel in CELLview™ dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes. ITEM AT GREINER'S WEBSITE

Description
Item No.:	627960
Product description:	CELLVIEW CELL CULTURE DISH, PS, 35/10 MM, VENTS, ADVANCED TC, STERILE, 10 PCS./BAG
Packaging weight:	2,80 kg
Packaging dimension:	295 x 195 x 370 mm
Packing unit:	740
Quantity per inner pack:	10
Sterile:	sterile

Detailed information

Advanced TC™ - Cell Culture Dish

• Advanced TC™ polymer modification for fastidious or sensitive adherent cells
• Free of detectable DNase, RNase, human DNA
• Non-pyrogenic, non-cytotoxic
• Advantages:

• Diameter: 35 mm; height: 10 mm
• Growth area: 8.7 cm²
• Total Volume: 10 ml
• Working volume: 5 ml
• With vents 
• Sterile

 

Quantity per bag/case: 10/740

ITEM AT GREINER'S WEBSITE

Description
Item No.:	627965
Product description:	CELLVIEW CELL CULTURE DISH, PS, 35/10 MM, GLASS BOTTOM, 1 COMPARTMENT, ADVANCED TC, STERILE, 10 PCS./BAG
Packaging weight:	0,40 kg
Packaging dimension:	195 x 142 x 122 mm
Packing unit:	40
Quantity per inner pack:	10
Sterile:	sterile

Detailed information

CELLview™ - Cell Culture Dish with Glass Bottom

• Free of detectable DNase, RNase, human DNA
• Non-pyrogenic, non-cytotoxic
• Glass bottom features:

                      - High transparent achromatic
                         borosilicate glass; hydrolytic
                         class 1 (DIN ISO 719)
                     - Glass thickness 175 µm +/- 15 µm
                     - Maximal spectral transmission; no
                       autofluorescence 

• Advantages:

                       -Subdivided version enables
                        simultaneous multiplex analysis
                      - Embedded glass bottom for maximal
                        planarity

• Number of compartments: 1
• Diameter: 35 mm; height: 10 mm
• Growth area: 8.7 cm²
• Total volume: 10 ml
• Working volume: 5 ml
• Surface treatment: Advanced TC™
• Sterile

 

Quantity per bag/case: 10/40

 

Videos
Drug treatment during live cell imaging
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:

• Video 1 - control (no drugs added)
  In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.
  
  
• Video 2 - Nocodazole added, final concentration 10 µM
  Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.
  
  
• Video 3 - Latrunculin B added, final concentration 1 μM
  Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi
  rearrangement was completed.
  
  
• Video 4 - Brefeldin A added, final concentration 5 μg/ml
  Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.

Performing these experiments in parallel in CELLview™ dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes. ITEM AT GREINER'S WEBSITE

Description
Item No.:	627975
Product description:	CELLVIEW CELL CULTURE DISH, PS, 35/10 MM, GLASS BOTTOM, 4 COMPARTMENTS, ADVANCED TC, STERILE, 10 PCS./BAG
Packaging weight:	0,40 kg
Packaging dimension:	195 x 142 x 122 mm
Packing unit:	40
Quantity per inner pack:	10
Sterile:	sterile

Detailed information

CELLview™ - Cell Culture Dish with Glass Bottom

• Free of detectable DNase, RNase, human DNA
• Non-pyrogenic, non-cytotoxic
• Glass bottom features:

                      - High transparent achromatic
                         borosilicate glass; hydrolytic
                         class 1 (DIN ISO 719)
                      - Glass thickness 175 µm +/- 15 µm
                      - Maximal spectral transmission; no
                         autofluorescence 

• Advantages:

                      -Subdivided version enables
                        simultaneous multiplex analysis
                     - Embedded glass bottom for maximal
                        planarity

• Number of compartments: 4
• Diameter: 35 mm; height: 10 mm
• Growth area: 1.9 cm²/compartment
• Total volume: 1.5 ml/compartment
• Working volume: 0.1 ml for seeding or staining only on glass area; 0.5 ml for cultivation in the complete compartment
• Surface treatment: Advanced TC ™
• Sterile

 

Quantity per bag/case: 10/40

Videos
Drug treatment during live cell imaging
A multi-position time-lapse experiment was started and after acquiring six time points every two minutes drugs were added to the different wells as indicated:

• Video 1 - control (no drugs added)
  In steady-state the Golgi apparatus is relatively stable on light microscopy level. The shape changes only slowly during the time of the experiment when observing control cells. Also the number of Golgi fragments visible by light microscopy resolution is relatively constant over time.
  
  
• Video 2 - Nocodazole added, final concentration 10 µM
  Nocodazole treatment induces, fragmentation of the Golgi apparatus. The onset of fragmentation starts 10 to 15 minutes after addition of the drug. The onset of fragmentation differs between individual cells. Fragmentation of the central Golgi to many distributed ministacks is the final phenotype of microtubule depolymerization after three hours.
  
  
• Video 3 - Latrunculin B added, final concentration 1 μM
  Actin depolymerization by Latrunculin B influences the shape of the Golgi from relatively thin elongated to a rounded up and compact appearance. After 10 to 20 minutes differences in the Golgi morphology became first visible and after approximately one hour the Golgi
  rearrangement was completed.
  
  
• Video 4 - Brefeldin A added, final concentration 5 μg/ml
  Block of export from the endoplasmatic reticulum (ER) by Brefeldin A leads to a rapid redistribution of the Golgi compartment to the ER by retrograde transport. This effect is often completed within 5 minutes.

Performing these experiments in parallel in CELLview™ dishes with four compartments it is possible to directly compare the speed and timing of drug effects on the Golgi apparatus. Brefeldin A affects Golgi morphology much faster than Nocodazole and Latrunculin B, which both induces first changes in the range of 10-20 minutes.
ITEM AT GREINER'S WEBSITE